As a part of the search for alternative methods that reduce the use of fertilizers and fungicides, the present work focused on the study of bio-stimulant and antifungal effects of two antagonist isolates collected from Algerian rhizosphere of tomato culture: T. asperellum and T. atroviride. The study was based on the evaluation of vegetative growth of tomato plants, yield of their fruit and the impact of their use on total polyphenol and foliar pigments contenents. Moreover, the biocontrol of downy mildew was assessed by in vivo antagonist activity on foliar discs of detached leaves of tomato against Phytophthora infestans (Mont.) de Bary. The results showed the biostimulant effect of these two isolates, particularly T. atroviride. The total polyphenols and leaf pigments contenents were significantly better on leaves of plants treated with T. atroviride. In addition, this study confirmed the antagonist activity of the two Trichoderma isolates against Phytophthora infestans, with the predominance of T. atroviride.